Abstract
To evaluate the effects of copaiba oil as a prophylactic and/or therapeutic substance on survival of rats subjected to cecal ligation and puncture, describing histopathological and oxidative stress findings. Forty rats (Ratus norvegicus) were distributed into five study groups (N=8): Sham group (ShG): normal standard animals; Sepse group (SepG): submitted a cecal ligation and puncture (CLP); Pre group (PreG): administered copaiba oil once daily by subcutaneous injection for five days before carrying out CLP; Post CLP group (PostG): administered copaiba oil once daily by subcutaneous injection from the first day of CLP until death by sepsis; and Pre/Post group (Pre/PostG): administered copaiba oil once daily by subcutaneous injection for five days before carrying out CLP and from the first day of CLP until de death by sepsis. After the death of the animals, blood was collected for assessment of oxidative stress and histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. Survival analysis demonstrated that animals treated with copaiba oil prior to the execution of the CLP (PreG and Pre/Post groups) had longer survival compared to the sepsis group (p<0.0001) whereas animals receiving copaiba only after the completion of CLP (PostG) showed no statistically significant difference compared to the sepsis group. However, when comparing the two groups in which was administered copaiba previously (PreG and Pre/PostG groups), there was no statistical significance between the groups (p=0.4672). There was no statistical difference between histopathological findings or the levels of oxidative stress. Prophylactic subcutaneous administration of copaiba increases survival of rats subjected to severe sepsis by cecal ligation and puncture.
Highlights
Peritonitis is one of the most frequent causes of sepsis and death in intensive care units
Survival analysis demonstrated that animals treated with copaiba oil prior to the execution of the cecal ligation and puncture (CLP) (PreG and Pre/Post groups) had longer survival compared to the sepsis group (p
Prophylactic subcutaneous administration of copaiba increases survival of rats subjected to severe sepsis by cecal ligation and puncture
Summary
Peritonitis is one of the most frequent causes of sepsis and death in intensive care units. Sepsis occurs when an intra-abdominal infection site triggers a systemic response[1]. Sepsis is a complex syndrome related to a systemic inflammatory response with multiple manifestations that may cause the dysfunction or failure of one or more organs or even death[2]. Severe sepsis and septic shock are major healthcare problems, affecting millions of people around the world each year[3], In the United States, severe sepsis is recorded in 2% of patients admitted to the hospital, The number of cases in the United States exceeds 750.000 per year and was recently reported to be rising[4], resulting in 215.000 deaths per year. The mortality rate for patients with SIRS (from either sepsis or any other cause), sepsis, severe sepsis and septic shock was 24.2%, 33.9%, 46.9% and 52.2%, respectively[5]
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