Copaiba oil effect under different pathways in mice subjected to sepsis.

Nara Macedo Botelho,Edvaldo Lima Silveira,Renan Kleber Costa Teixeira,Felipe Augusto Folha Santos,Letícia Nobre Lopes,Thaís Travassos Da Silva

doi:10.1590/s0102-86502014000800008

Nara Macedo Botelho, Edvaldo Lima Silveira + Show 4 more

Open Access

https://doi.org/10.1590/s0102-86502014000800008

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Journal: Acta cirurgica brasileira	Publication Date: Aug 1, 2014
Citations: 6	License type: cc-by

Affiliation: Universidade do Estado do Pará

Abstract

To evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture. Thirty two mice were distributed into four study groups (N=8): Sham group: normal standard animals; submitted a cecal ligation and puncture (CLP); Gavage group: submitted a CLP, and treat with copaiba oil by gavage; and Subcutaneous group: submitted a CLP, and treat with copaiba oil by subcutaneous injection. After the death of the histological analysis were performed. The Kaplan-Meier curves of surviving time were realized. All animals that received copaiba, regardless of the route used, survived longer when compared to the control group (p<0.0001), whereas the survival time ranged from 20 hours for the control group up to 32 hours for the animals of gavage group and 52 for subcutaneous group. The animals that received gavage copaiba lived about and about 20 hours unless the subcutaneous group (p=0.0042). There was no statistical difference when compared the intensity of inflammatory response (p>0.05) CONCLUSION: Prophylactic subcutaneous administration of copaiba in mice subjected to severe sepsis by cecal ligation and puncture, resulted in a survival time higher than non-use or use of this oil by gavage.

Highlights

Sepsis is the leading cause of death in intensive care units (ICU) with a mortality rateranging from 20 to 80%, being diagnosed 18 million new cases of severe sepsis per year worldwide[1,2,3,4].The incidence of severe sepsis increased 91.3% over the past 10 years occurring in 1 to 3 per 1000 individuals in different regions, with annual growth of 1% per year[1,5], representing still a severe public health problem in the world, both from the point of view of social and economic terms[6]
The treatment of sepsis represents a challenge for medicine[7], because it still remains an entity of difficult clinical management
The aim of this study is to evaluate the effects of copaiba oil administered by different routes on survival of mices subjected to cecal ligation and puncture

Summary

Introduction

Sepsis is the leading cause of death in intensive care units (ICU) with a mortality rateranging from 20 to 80%, being diagnosed 18 million new cases of severe sepsis per year worldwide[1,2,3,4].The incidence of severe sepsis increased 91.3% over the past 10 years occurring in 1 to 3 per 1000 individuals in different regions, with annual growth of 1% per year[1,5], representing still a severe public health problem in the world, both from the point of view of social and economic terms[6]. Along with the United Kingdon, as one of the countries with the highest rate of occurrence of sepsis in ICU, responsible for occupying about one quarter of the ICU beds[3]. The treatment of sepsis represents a challenge for medicine[7], because it still remains an entity of difficult clinical management. The use of plants for medicinal purposes, for treatment, prevention and cure of diseases, is one of the oldest forms of medical practice of mankind. In the early 1990s, the World Health Organization reported that 65-80% of the population in developing countries depended on medicinal plants as the only form of access to basic health care[9], and in relation to world population, about 60% makes use of medicinal plants[10]

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