Effect of hydroxyethyl starch 130/0.4 solutions with different volume on coagulant function in rats with severe sepsis

Abstract

Aim to determine the effect of different dose of 6% 130/0.4 hydroxyethyl starch on coagulation function in rats model with severe sepsis by cecal ligation and puncture (CLP). 80 male SD rats were selected and randomly divided into the sham groups and the CLP groups, every group was divided into 4 small Groups again: before recovery(H0), 7.5 ml/kg group(H1), 15 ml/kg group (H2) and 30 ml/kg group(H3), n = 10; The CLP groups were established by cecal ligation and perforation (CLP), the sham groups were the same as the CLP groups except for CLP, MAP was monitored for all rats during the experiment. After 4 hours by CLP , Procedure fluid resuscitation which received 6% hydroxyethyl starch 130/0.4 by respectively 7.5 ml/kg, 15 ml/kg, 30 ml/kg were started at a rate of 10 ml·kg(-1)·h(-1), the sham groups were the same dose 6% hydroxyethyl starch 130/0.4 at the same time. All rats were taken for arterial blood harvest at 4 h after fluid infusion for arterial blood gases, blood routine examination, conventional coagulation function and thrombelastography (TEG). Compared with the sham groups, PO2 in the CLP groups was not significantly different.pH, PCO2 and MAP were significantly lower than the sham groups, Lac was significantly higher than the sham groups (P < 0.05). APTT and PT were significantly higher than the sham groups (P < 0.05), D-Dime and FIB were not significant different (P > 0.05), R and K were significantly shorter than the sham groups, MA was significantly higher than the sham groups (P < 0.05), α and CI were higher than the sham groups, but the difference was not statistically significant (P > 0.05).Within CLP group, compared with H0, R, K, α, MA and CI after fluid resuscitation were not significant different. Low-dose (less than 30 ml/kg) 6% hydroxyethyl starch 130/0.4 in early fluid resuscitation have less effect on coagulation function in rats with severe sepsis.