Abstract
This chapter discusses the cooperation requirements of mitogen-stimulated lymphocytes in agarose-gel cultures. Lymphocytes capable of proliferation in solid and liquid cultures appear to be subpopulations qualitatively different from lymphocytes capable of proliferation in liquid cultures only. The chapter presents a study in which the analysis of lymphocyte stimulation kinetics, developed by Coppleson and Michie, was used to investigate a possible requirement for cell interactions in lymphocyte stimulation by mitogens in solid cultures. In such an approach, each cell is considered a kinetic particle, and consequently, the order of kinetics would be indicative of the number of cell interactions required to obtain a mensurable event. The order of kinetics is indicated by the value of regression coefficients of a line obtained after plotting on a logarithmic scale, the different lymphocyte concentrations per culture in the abscissa, and the magnitude of their respective responses in the ordinate. In the study described in the chapter, the possibility of the existence of qualitatively different cooperative mechanisms was explored. The role of erythrocytes as a source of cooperative signals was also studied.
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