Abstract
The present study was planned to investigate equine babesiosis in dragging horses in Baghdad city, Iraq by using microscopical and molecular (PCR) techniques. 150 blood samples of horses examined for Theileria equi and Babesia caballi. 16.66% (25/150) were positive by microscopic examination. No significant difference was observed in infection rates between male and female horses and among different age groups. The result showed that PCR method has high rate of infection36% (9/25). Nine positive PCR products were sequenced and deposited in Genebank data base for first time in Iraq, phylogenic analysis demonstrated that 5 sequences belongs to T. equi (MK350319, MK346272, MK346273, MK346274 and MK36275), while 4 sequence (MK346276, MK346277, MK346278 and MK350318) belongs to B. caballi, and mounted a low genetic variation 0.035 and 0.05 respectively, among other comparison isolates. In conclusion PCR technique followed by phylogenic tree analysis a reliable methods for epidemiological, diagnosis and identification of genetic variants studies.
Highlights
R method has high rate of infection36% (9/25)
Parasitic tick-borne disease affecting equids Microscopic examination, it is Thin smears of the blood smears were produced by Babesia spp. (B. prepared from the first part 2 ml of blood caballi and Theileria equi ) protozoan [12,13]. samples collected from animals according to Babesia species are transmitted by tick species [9]
Causative samples microscope; this technique has the used in DNA extraction purpose according to disadvantage of being having low sensitivity, commercial purification method described by mostly in horses with low parasitemia [14]
Summary
Equine babesiosis as the most important blood College- University of Baghdad. parasitic tick-borne disease affecting equids Microscopic examination (horse, pony, donkey, and mule), it is Thin smears of the blood smears were produced by Babesia spp. (B. prepared from the first part 2 ml of blood caballi and Theileria equi ) protozoan [12,13]. samples collected from animals according to Babesia species are transmitted by tick species [9]. Causative samples (collected randomly from 150 blood agent of equine babesiosis can be detected by sample examined previously by microscope) microscope; this technique has the used in DNA extraction purpose according to disadvantage of being having low sensitivity, commercial purification method described by mostly in horses with low parasitemia [14]. Baghdad city, during from January to July reaction volume of (25μL), Master Mix (5μl), 2018, blood samples was taken from the DNA template(1.5μl), (1μl) of each jugular vein from horses of different breed, primer, 5μl)D.W. PCR conditions ages and of sexes, 5 ml blood samples were start with initial denaturation(94oC for 5 min), collected from each animal and was divided denaturation(94oC 45min), A 35 cycle of into two parts, the first (2 ml) of the blood annealing 58oC for first primer and 60oC for used immediately for blood smears stained second primer for 45 min, extension(72oC for with Giemsa stain, and the rest (2-3ml )of 45min) with final extension of(72oC for 5 blood was collected in EDTA tube, for DNA min)
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