TRADITIONAL AND MOLECULAR STUDY OF CRYPTOSOPORIDIUM SPP. IN DOMESTIC DOGS IN BAGHDAD CITY,IRAQ

A A Faraj

doi:10.36103/ijas.v50i4.754

Abstract

The aim of this study to investigate the prevalence of Cryptosoridium spp. in domestic dogs in Baghdad city, by using microscopic examination(flotation and staining) and molecular techniques .The results revealed that the rate of Cryptosporidium infection was 15% by microscopic examination and 28.6% using PCR, our study showed that the infection rate was 8.5% in males and 21.42% in females by microscopic examination , while the infection rate by using PCR in males and females was 21.42%, 35.71% respectively. The percentage of infection in the age groups <1 year and >1 year was 20%, 10% respectively by microscopic examination, while by using molecular assay the rate of infection in the age groups <1 year was 40% and 17.14% in >1 year. This is the first study in Iraq to detect Cryptosoridium spp. in domestic dogs using molecular technique.

Highlights

‫ وذلك باستخدام الفحص‬، ‫هدفت الد ارسة الحالية للتحري عن انتشار الابواغ الخبيئة في الكلاب المنزلية في مدينة بغداد‬
Prof. *Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Iraq aazhar888@yahoo.com ABSTRACT The aim of this study to investigate the prevalence of Cryptosoridium spp. in domestic dogs in Baghdad city, by using microscopic examination(flotation and staining) and molecular techniques .The results revealed that the rate of Cryptosporidium infection was 15% by microscopic examination and 28.6% using PCR, our study showed that the infection rate was 8.5% in males and 21.42% in females by microscopic examination, while the infection rate by using PCR in males and females was 21.42%, 35.71% respectively
Infection is One hundred and forty fecal samples (10 g) occur via the fecal–oral route after the from domestic dogs, of different sexes and ingestion of contaminated food or water with ages referred that a small animal private oocysts [17, 3], Diarrhea is the most important clinics in Baghdad, Iraq

Summary

INTRODUCTION

Conducted to determine the prevalence and Cryptosporidiosis is one of the important a molecular diagnosis Cryptosporidium species parasitic diseases , caused by in domestic dogs in Baghdad city, Iraq. Infection is One hundred and forty fecal samples (10 g) occur via the fecal–oral route after the from domestic dogs, of different sexes and ingestion of contaminated food or water with ages referred that a small animal private oocysts [17, 3], Diarrhea is the most important clinics in Baghdad, Iraq. Diagnoses at 2.5% potassium dichromate (K2Cr2O7) [12], Cryptosporidium of infection in fecal samples and stored at (-20 C °) until DNA was of dogs is based by identifying the oocysts extracted. Design this sequencing analysis to define the species of primer by the (NCBI)gene-Bank data the parasite [13,2]. PCR the data was done by using SAS software) was done and showed 300 bp band which

RESULTS AND DISCUSSION

Observer B

Chi square value

Phylogenetic Analysis recorded under accession number

This refers that sex is not a major affecting