Abstract
Ascaridiosis is a very important parasitic disease of birds, it is caused by Ascaridia. This study was conducted to identify the Ascaridia species by microscopic and molecular assay in Baghdad city. One hundred and sixty fecal samples were collected from domestic pigeons during the period from 1/1/ 2019 to 31/3/ 2019. Results showed that the rate of infection for Ascaridia spp. 15.62% by microscopic examination. Significant difference was observed in infection rates between males and females pigeons. Fifty samples randomly selected and subjected to molecular diagnosis of Ascaridia spp.. Molecular examination results, the total infection rate showed 16%(8/50). The eight positive PCR products were sequenced and deposited in Gene bank data base, phylogenic analysis demonstrated that 4 sequences belongs to Ascaridia galli ( MK918635.1, MK918636.1, MK918847.1, MK919081.1), while 2 (MK919199.1, MK919200.1) belong to Ascaridia nymphii and 2 (MK919207.1, MK919264.1) belong to Ascaridia numidae. It is the first study in Iraq to diagnosis of Ascaridia nymphii and Ascaridia numidae in domesticed pigeons by using conventional PCR.
Highlights
R Ascaridia spp. 15.62% by microscopic examination
Significant difference was observed in infection rates between males and females pigeons
The eight positive PCR products were sequenced and deposited in Gene bank data base, phylogenic analysis demonstrated that 4 sequences belongs to Ascaridia galli ( MK918635.1, MK918636.1, MK918847.1, MK919081.1), while 2 (MK919199.1, MK919200.1) belong to Ascaridia nymphii and 2 (MK919207.1, MK919264.1)
Summary
Birds may harbour numerous intestinal Baghdad.Fecal samples were removed parasites, including protozoa, cestodes, from each pigeons into a dry petri dish from trematodes and nematodes [8;19]. Ascaridiosis caused by Ascaridia is a most microscopic examination (direct wet smear) common nematoda worm in domestic birds. Ascaridia has a direct life cycle, The eggs of described according [21], the second part kept. The released larvae can Molecular diagnosis Method cause destruction and erosion of the intestinal Genomic DNA extraction: Fifty fecal mucosal layer and proliferation of mucus samples collected randomly from 160 feacal secreting cells [13]. Ascaridia infections are sample used in DNA extraction purpose often associated with reduced body condition, according to commercial purification method increased feed conversion ratio, and overall described by G- spin DNA extraction kit reduced health conditions [5].The infection cat.no. MATERIALS AND METHODES and integrity [7; 10]
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