Abstract
A method was developed for the continuous measurement of α-glucans in weak alkaline eluates from the gel permeation chromatography of the components of entire or debranched starch. A modified flow injection analysis system was used comprising the continuous neutralization of the alkali with dilute acid, the hydrolysis of α-glucans with amyloglucosidase and the in-flow spectrophotometric determination of the resulting glucose with an improved glucose oxidase-peroxidase-chromogen reagent. The system is sensitive, reliable and safe compared with hitherto used colorimetric methods relying on hot concentrated sulphuric acid.
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