Construction of ratiometric fluorescent nanosensors based on carbon dots dual emission strategy for high-sensitivity visual detection of Ag+

Abstract

ABSTRACT Water-soluble purple fluorescent carbon nanodots (P-CDs) were synthesised using 2-phenylphenol, and red fluorescent carbon dots (R-CDs) were prepared from o-phenylenediamine and phosphoric acid. Based on the fluorescence quenching effect of Ag+ on carbon quantum dots, and additionally introducing a red light-emitting quantum dot with excellent performance and high quantum yield, a dual emission ratiometric fluorescent nanosensor (P-CDs/R-CDs) was established. The semi-quantitative detection of silver ions can be realised. Under the excitation light of 254 nm and 365 nm, the fluorescence spectrum shows double emission fluorescence peaks at 343 nm and 626 nm, which originate from the fluorescence emission of the synthesised C-dots and the additionally introduced C-dots, respectively. The relationship between the ratio of the intensity of the two emission peaks F343/F626 and the concentration of silver ions realises the analysis and detection of silver ions. The linear detection range of this method was 0.096–10 μM, and the detection limit (LOD, S/N = 3) was 32 nM. In addition, the method can also perform naked-eye visual detection according to the fluorescence colour change of the probe under ultraviolet light, and realise the rapid monitoring of Ag+ on site. The P-CDs/R-CDs ratiometric fluorescence sensor provides a new method for the detection of Ag+ and promotes the development of heavy metal ion sensing.