Abstract
Prox1 is involved in muscle fiber conversion, adult-onset obesity, and type 2 diabetes. However, information regarding porcine Prox1 and its relationship with meat quality traits is still unknown. In this study, we characterized the full-length cDNA and proximal promoter of two transcript variants of porcine Prox1. Moreover, Prox1 was expressed abundantly in the skeletal muscle and its expression was higher in the soleus muscle than that in the biceps femoris muscle. Its expression pattern in the high and low meat color (redness) value a* groups was similar to that of myoglobin and MyHC I, but opposed to that of MyHC IIB. Importantly, there was a significant positive correlation between Prox1 expression and meat color (redness) value a* (r = 0.3845, p = 0.0394), and a significant negative correlation between Prox1 expression and drip loss (r = -0.4204, p = 0.0232), as well as the ratio of MyHC IIB to MyHC I expression (r = -0.3871, p = 0.0380). In addition, we found that the polymorphisms of three closely linked SNPs in Prox1 promoter 1 were significantly associated with pH24h in a pig population. Taken together, our data provide valuable insights into the characteristics of porcine Prox1 and indicate that Prox1 is a promising candidate gene affecting meat quality traits.
Highlights
Prospero-related homeobox 1 (Prox1) encodes a homeobox transcription factor in vertebrates
Two kinds of alternative splicing forms were found in the 50 -end sequence of Prox1
We deduced that at least two putative transcript variants exist in porcine Prox1
Summary
Prospero-related homeobox 1 (Prox1) encodes a homeobox transcription factor in vertebrates. Previous studies have demonstrated that Prox is essential for the embryonic development of the liver [1], lymphatic system organs [2,3], pancreas [4], lens [5], and retina [6]. Prox plays an important role in regulating hepatic triglycerides levels by mediating the HDAC3-HNF4α pathway [7], indicating that Prox is critical for adipogenesis and fat storage. Recent studies have shown that Prox plays a pivotal role in heart development [8] and skeletal muscle fiber conversion [9,10,11]. We detected differentially expressed genes (DEGs) between the classic red muscle (soleus muscle) and white muscle (biceps femoris muscle) with various muscle fibers, and identified
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