Abstract
The composition, template activity in RNA-polymerase systems of RNA-synthesis and thermal stability of rat liver chromatin in animals of various age (1, 3, 12 and 30 months) were investigated after deproteinization with NaCl solutions of growing concentrations. Dissociation changes of arginine-rich histones were seen in 30 months old rats, testifying to a strengthening of those protiens' bonds with DNA. With age, there is also an increase in the thermostability of the chromatin proteinic fraction containing residual histones. The drop in template activity of old (30 months) rat liver chromatin in the RNA-polymerase system of RNA synthesis is associated predominatingly with changes in the protein fraction which dissociates at the interval of 1·25-2·0 M NaCl, where a strengthening of histones-DNA bonding is observed, as well as a decrease in the acid proteins. The increase in template activity of old rat liver chromatin in proportion to NaCl concentration increase proceeds much slower than in the chromatin of young (1 month old) rats.
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