Complete genomic sequence analysis of a GV Murine Norovirus in wild mice

Abstract

0bjective To identify the molecular structure and genome homology characteristics of a GV murine norovirus (MNV) from wild mice. Methods Nucleic acids were extracted from collected mouse intestinal specimens and subjected to high-throughput sequencing. The whole genome sequence was obtained by RT-PCR and RACE method . Results The MNV genome was 7, 832 bp in length, with 76.8%-78.1% nucleotide identity to other selected murine norovirus strains in GenBank. The P2 region, the hypervariable area in viral protein 1 gene shows highest heterology, and the identity was among 60.9%-78.1%, lower than other genes regions. The result demonstrated the higher variability of this newly isolated WNV strain. Conclusions The wild-type MNV isolated in this study is first reported in China, which enriched the domestic MNV gene pool and provided a reference for understanding the molecular genetic characteristics and evolutionary origin of MNV in China. Key words: Murine Noroviruses (MNV); Whole genome; Phylogenetic analysis