Abstract
Previously, Rhodobacter sphaeroides strain HJ was isolated to obtain a purple nonsulfur bacterium with the ability to produce high levels of hydrogen from acetate. However, the genome of this strain has not been previously sequenced. Therefore, the complete genome sequence of R. sphaeroides strain HJ is presented in this report.
Highlights
Rhodobacter sphaeroides strain HJ was isolated to obtain a purple nonsulfur bacterium with the ability to produce high levels of hydrogen from acetate
After the whole genome was assembled, gene functions were annotated via the NCBI Prokaryotic Genome Annotation Pipeline
The assembled genome of R. sphaeroides strain HJ comprises two chromosomes and a single plasmid in three contigs, and the overlapping ends of these chromosomes and plasmid were manually removed to finish circularization, resulting in two circular chromosomes designated chromosome 1 (3,263,112 bp with about 234-fold coverage) and chromosome 2 (1,186,739 bp with about 227-fold coverage) and a single plasmid (96,568 bp with about 97-fold coverage)
Summary
Rhodobacter sphaeroides strain HJ was isolated to obtain a purple nonsulfur bacterium with the ability to produce high levels of hydrogen from acetate. Complete genome sequence of Rhodobacter sphaeroides strain HJ, a purple nonsulfur bacterium with the ability to produce high levels of hydrogen from acetate. Kobayashi and Kondo bp; target coverage, 25; overlapper error rate, 0.06; overlapper minimum length, 40 bp; overlapper k-mer, 14), BLASR v1 (maximum divergence, 30; minimum anchor size, 12), and AssemblyPolishing v1 (use only unambiguously mapped reads, true) were used for filtering, assembling, mapping, and consensus polishing, respectively.
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