Abstract
The sodium/calcium exchanger-like (NCL) transporters are members of Ca2+/Cation antiporters (CaCAs) family, localized at the tonoplast, and primarily involved in Ca2+ homeostasis and stress response. They transport Ca2+ to the cytosol and sequester cytosolic Na+ into the vacuole. Therefore, the atncl mutant of Arabidopsis thaliana is prone to salinity stress. The functional complementation of TaNCL2-A of Triticum aestivum improved abiotic stress response and various morpho-physio-biochemical parameters in atncl mutant. The TaNCL2-A complementation increased the seed germination rate and root length of atncl mutant during salinity and drought stress conditions. The exogenous Ca2+ application further improved the stress tolerance in the complemented lines. The results suggested that the modulation of cytosolic Ca2+ by TaNCL2-A expression and/or exogenous Ca2+ application could reinstate growth and abiotic stress response in atncl mutant. TaNCL2-A also reduced the impact of ABA on seed germination. In addition, exogenous IAA induced lateral roots formation in all the lines. Biochemical and physiological analyses revealed increased proline, chlorophylls, carotenoids and relative water content (RWC), and reduced malondialdehyde (MDA), H2O2 and relative electrical conductivity (REC) in TaNCL2-A complemented lines. The results highlighted the function of TaNCL2-A gene in stress response, and its potential application in crop improvement strategies in future studies.
Published Version
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