Abstract
1. 1. To investigate the extent to which some commonly used differential inhibitors of isolated eukaryotic DNA polymerases retain their specificity of action in complex DNA-synthesizing cell-free systems a direct comparison was made of the effects they have on [ 3H]dTTP incorporation in isolated rat liver nuclei. 2. 2. Aphidicolin and araCTP inhibited DNA polymerase α but not DNA polymerase β. 3. 3. N-ethylmaleimide inhibited α and β when the activity of the two enzymes depended upon an exogenous primed template. 4. 4. The activity of the chromatin template dependent DNA polymerase β was either directly or indirectly sensitive to the sulfidryl-group-blocking agent. 5. 5. ddTTP did not manifest any selective inhibition of DNA polymerase β. DNA polymerase α showing an equally high sensitivity to the dTTP analog. 6. 6. Isolated regenerating rat liver nuclei do not offer a highly reliable DNA replication system in vitro.
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