The inhibitory action of pyrrolidine alkaloid, 1,4-dideoxy-1,4-imino- d-ribitol, on eukaryotic DNA polymerases

Abstract

The pyrrolidine alkaloids mimicking the structures of pentose with nitrogen in the ring are known to be inhibitors of glycosidases. We report here that a compound belonging to this category is an inhibitor of eukaryotic DNA polymerases. Among the eight naturally occurring pyrrolidine alkaloids we tested, only one compound, 1,4-dideoxy-1,4-imino- d-ribitol (DRB), which was purified from the mulberry tree ( Morus alba), strongly inhibited the activities of eukaryotic DNA polymerases with IC 50 values of 21– 35 μM , and had almost no effect on the activities of prokaryotic DNA polymerases, nor DNA metabolic enzymes such as human immunodeficiency virus type 1 reverse transcriptase, T7 RNA polymerase, and bovine deoxyribonuclease I. Kinetic studies showed that inhibition of both DNA polymerases α and β by DRB was competitive with respect to dNTP substrate. Whereas DNA polymerase α inhibition was noncompetitive with the template-primer, the inhibition of DNA polymerase β was found to be competitive with the template-primer. The K i values of DNA polymerases α and β for the template-primer were smaller than those for dNTP substrate. Therefore, the affinity of DRB was suggested to be higher at the template-primer binding site than at the dNTP substrate-binding site, although DRB is an analogue of deoxyribose consisting of dNTP. Computational analyses of the eight pyrrolidine alkaloids revealed a remarkable difference in the distribution of positive and negative electrostatic charges on the surface of molecules. The relationship between the structure of DRB and the inhibition of eukaryotic DNA polymerases is discussed.