Comparison of the Effect of Two types of High-Intensity Interval Training on the Expression Genes of IRS-1 and IRE-1α in the Cardiomyocytes type 2 diabetes of Obes Male Rats

Abstract

Background: The purpose of this study was Comparison Comparison the Effect of Two types of High-Intensity Interval Training on the Expression Genes of IRS-1 and IRE-1α in the Cardiomyocytes type 2 diabetes of Obes Male Rats. Material and Methods: 36 male Wistar rats were divided into 4 groups: type1 high-intensity interval training (HIIT1:1), type2 high-intensity interval training (HIIT2:1), diabetic control (DC), and nondiabetic control (NC). Plasma glucose concentration was measured by the glucose oxidase method. ELISA method was used to measure the insulin levels and the HOMA-IR method was used to measure insulin resistance index. A real-time PCR technique was used to evaluate the gene expression of the IRS-1 and IRE-1α genes. Results were analyzed with one-way ANOVA and Tukey post hoc tests at the alpha level of 0/05.Results: gene expression of IRS-1 in both groups of HIIT2:1(p= 0.002) and HIIT1:1(p= 0.006) showed a significant decrease compared to the DC group and in HIIT2:1(P=0/043) showed a significant decrease compared to the HIIT1:1 group. a gene of IRE-1α in both groups of HIIT2:1(p= 0.005) and HIIT1:1(p= 0.007) showed a significant decrease compared to the DC group and in HIIT2:1(P=0/001) showed a significant decrease compared to the HIIT1:1 group. The mean weight values and insulin resistance index in the HIIT1:1 group and glucose were significantly lower in the HIIT2: 1 group.Conclusion: High-intensity interval training type 2 with a higher effect on decreasing IRS-1 and IRE-1α genes expression in cardiomyocytes of obese diabetic rats may it can improve gene dysregulation.