Abstract
This study investigated whether there are marked differences in surface markers between rabbit and human mesenchymal stem cells (MSCs). Murine and rabbit MSCs have been reported to be CD90-negative. Rat MSCs have been reported to be CD71-negative. Our previous study also shows that rabbit MSCs are CD29-negative. However, human MSCs are generally considered to be CD29-, CD71-, and CD90-positive. Therefore, the surface markers of human MSCs might differ from those of other species. Rabbit bone marrow MSCs were obtained that had a multi-differentiation potential. The phenotype of these cells was studied using flow cytometry antibodies for 25 rabbit surface markers, namely, CD13, CD14, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD51, CD54, CD59, CD71, CD73, CD90, CD105, CD106, CD133, CD166, MHC I, MHC II, α-smooth muscle actin (α-SMA), cytokeratin, desmin, and vimentin. The phenotype of commercially available human MSCs was similarly studied using antibodies for human surface markers. CD14, CD31, CD34, CD45, CD49d, CD49f, CD51, CD54, CD71, CD106, CD133, MHC II, and cytokeratin were absent from both rabbit and human MSCs, while CD44, α-SMA, and vimentin were present on both cell lines. CD13, CD29, CD59, CD73, CD90, CD105, CD166, and MHC I were present on human MSCs, but not on rabbit MSCs. However, desmin was present on rabbit MSCs, but not on human MSCs. In total, the surface expression of nine markers differed between human and rabbit MSCs, whereas the surface expression of 16 markers was the same in the two cell lines.
Highlights
Biological and clinical interest in mesenchymal stem cells (MSCs) has increased dramatically over the past two decades [1,2]
Our results showed that CD14, CD31, CD34, CD45, CD49d, CD49f, CD51, CD54, CD71, CD106, CD133, MHC II, cytokeratin, and desmin were absent from human MSCs, whereas CD13, CD29, CD44, CD59, CD73, CD90, CD105, CD166, MHC I, a-smooth muscle actin (a-SMA), and vimentin were present on human MSCs
CD14, CD31, CD34, CD45, CD49d, CD49f, CD51, CD54, CD71, CD106, CD133, MHC II, and cytokeratin were absent from both rabbit and human MSCs, whereas CD44, a-SMA, and vimentin were present on both cell lines
Summary
Biological and clinical interest in mesenchymal stem cells (MSCs) has increased dramatically over the past two decades [1,2]. MSCs are multipotent cells that can replicate and have the potential to differentiate into cells of mesenchymal lineages, including bone, cartilage, and fat [2]. According to a report by Pittenger et al, human MSCs are characterized by the presence of particular marker proteins on their surface, including CD29, CD44, CD71, CD90, and CD105, and by the absence of marker proteins of leukocytes and cells of hematopoietic lineage, including CD14, CD34, and CD45 [2]. Our previous study showed that rabbit MSCs are CD29- and CD90-negative [6]. Martınez-Lorenzo et al found species-related differences in the phenotypes of MSCs from human, rabbit, and sheep [7]
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