Abstract
BackgroundThe surface markers of mesenchymal stem cells (MSCs) of rabbits have been reported only sporadically. However, interest in the spinal fusion effect of MSCs has risen recently. The purpose of this research was to study the surface markers and spinal fusion effect of rabbit MSCs.ResultsOf our rabbit MSCs, 2% expressed CD14, CD29, and CD45, 1% expressed CD90 and 97% expressed CD44. These results implied the MSCs were negative for CD14, CD29, CD45, and CD90, but positive for CD44. The surgical results showed that satisfactory fusion occurred in 10 rabbits (83%) in the study group and unsatisfactory fusion in 2 (17%). In the control group, satisfactory fusion was found in 3 rabbits (25%) and unsatisfactory fusion in 9 (75%). Statistical analysis showed the study group had significantly better spinal fusion results than the control group.ConclusionsThe surface markers of human and rabbit MSCs are not exactly the same. Rabbit MSCs do not have positive reactivity for CD29 and CD90, which are invariably present on human MSCs. The allogeneic undifferentiated rabbit MSCs were able to promote spinal fusion and did not induce an adverse immune response.
Highlights
The surface markers of mesenchymal stem cells (MSCs) of rabbits have been reported only sporadically
Isolation and culture of rabbit bone marrow MSCs Under an inverted microscope, minimal fibroblast-like cells were found adhering to the culture surface at 1 week after plating
The current study showed that 2% of our cells expressed CD14, CD29 and CD45, and 1% expressed CD90
Summary
The surface markers of mesenchymal stem cells (MSCs) of rabbits have been reported only sporadically. The purpose of this research was to study the surface markers and spinal fusion effect of rabbit MSCs. Biological and clinical interest in mesenchymal stem cells (MSCs) has risen dramatically over the past two decades [1,2]. Adult bone marrow contains a heterogeneous population of cells, including hematopoietic stem cells, macrophages, erythrocytes, fibroblasts, adipocytes, and endothelial cells. In addition to these cell types, bone marrow contains a subset of nonhematopoietic stem cells that possess a multilineage potential [3,4]. Human MSCs are characterized by the presence of a consistent set of marker
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