Abstract
Simple SummaryProgesterone has been proven to play an important role in female mammals during pregnancy. In this study, the uteri of pregnant mice were collected to compare mRNA and lncRNA expression between periods of embryo implantation and labor. The results show that 19 known differentially expressed lncRNAs and 31 novel differentially lncRNAs were commonly expressed between the two stages, indicating that these lncRNAs’ function is related to progesterone.Uterine function during pregnancy is regulated mainly by progesterone (P4) and estrogen (E2). Serum P4 levels are known to fluctuate significantly over the course of pregnancy, especially during embryo implantation and labor. In this study, pregnant mice at E0.5, E4.5, E15.5, and E18.5 (n = 3/E) were used for an RNA-Seq-based analysis of mRNA and lncRNA expression. In this analysis, 1971 differentially expressed (DE) mRNAs, 493 known DE lncRNAs, and 1041 novel DE lncRNAs were found between E0.5 and E4.5 at the embryo implantation stage, while 1149 DE mRNAs, 192 known DE lncRNAs, and 218 novel DE lncRNAs were found between E15.5 and E18.5 at the labor stage. The expression level of lncRNA-MMP11 was significantly downregulated by P4 treatment on MSM cells, while lncRNA-ANKRD37 was significantly upregulated. Notably, 117 DE mRNAs, 19 known DE lncRNAs, and 31 novel DE lncRNAs were commonly expressed between the two stages, indicating that these mRNAs and lncRNAs may be directly or indirectly regulated by P4.
Highlights
In female mammalian reproduction, crosstalk between the embryo and the uterus is present throughout pregnancy
Crosstalk becomes frequent during the embryo implantation stage and labor stage, and is regulated mainly by progesterone (P4)
The P4 level remains high to maintain uterine quiescence until E15.5, with progesterone receptor function decreasing (Figure 1A) [25,26,27]. Both stages are connected with an increased level of inflammatory response; this is characterized by increased production of proinflammatory cytokines such as IL-1β and IL6 (Figure 1B) [23]
Summary
Crosstalk between the embryo and the uterus is present throughout pregnancy. The P4 level remains high to maintain uterine quiescence until E15.5, with progesterone receptor function decreasing (Figure 1A) [25,26,27] Both stages are connected with an increased level of inflammatory response; this is characterized by increased production of proinflammatory [11,23,24]. Both stages are connected with an increased level of inflammatory response; this is characterized by increased production of proinflammatory cytokines such as IL-1β and IL6 (Figure 1B) [23]. Embryo implantation and labor ulated by changes in P4 via a similar molecular mechanism
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