Abstract
The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. The percentage agreement between the two methods, for all the isolates with < 2 dilutions that were tested was 91.6% for ketoconazole and griseofulvin, 88.3% for itraconazole, 81.6% for terbinafine and 73.3% for fluconazole. One hundred percent agreement was obtained for Trichophyton mentagrophytes isolates evaluated with ketoconazole and griseofulvin. Thus, until a reference method for testing the in vitro susceptibility of dermatophytes is standardized, the similarity of the results between the two methods means that the agar dilution method may be useful for susceptibility testing on these filamentous fungi.
Highlights
The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis
Telefax: 55 62 3521-1839 e-mail: rosario@iptsp.ufg.br Received in 10/10/2008 Accepted in 15/05/2009 by the lack of reliable in vitro techniques for determining the minimum inhibitory concentration (MIC) of antifungal agents against dermatophytes. Various methods, such as broth macro and microdilutions, agar dilution, Etest®, Sensititre® colorimetric microdilution panels and disk diffusion have been used for determining the susceptibility of dermatophytes to antifungal agents[7 12 13 14 15 16 19]
Dermatophytes were not included in the M38-A document, published by the Clinical and Laboratory Standards Institute in 20025, in which MICs for several antifungal agents against conidium-forming filamentous fungi are determined
Summary
The purpose of this study was to compare the agar dilution and broth microdilution methods for determining the minimum inhibitory concentration (MIC) of fluconazole, itraconazole, ketoconazole, griseofulvin and terbinafine for 60 dermatophyte samples belonging to the species Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. Research to evaluate in vitro susceptibility has been hampered Various methods, such as broth macro and microdilutions, agar dilution, Etest®, Sensititre® colorimetric microdilution panels and disk diffusion have been used for determining the susceptibility of dermatophytes to antifungal agents[7 12 13 14 15 16 19]. In order to compare such results, we used the agar dilution and broth microdilution methods for five antifungal agents against 60 dermatophyte strains belonging to three different species
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