Abstract
Two species of bacteria, identified as Pseudomonas stutzeri (P-16) and Pseudomonas saccharophila (P-15) by fatty acid methyl ester analysis, were found in a phenanthrene enrichment culture of a creosote-contaminated soil. The organisms are shown to be physiologically dissimilar, and their genetic relatedness is discussed. Phenanthrene degradation by both organisms followed Michaelis-Menten kinetics, allowing for the determination of half-saturation (Ks) and maximum activity coefficients, using nonlinear regression. Both organisms utilized kinetically similar enzymes for phenanthrene uptake and oxidation, as evidenced by similar Ks coefficients of approximately 0.2 mg/L and temperature optima of 40 degrees C, but levels of expression differed with different media. Each organism degraded phenanthrene via salicylic acid, but patterns of intermediate metabolism were shown to differ. P-15 excreted 1-hydroxy-2-naphthoic acid during growth on phenanthrene and demonstrated Michaelis-Menten kinetics for the oxidation of 1-hydroxy-2-naphthoic acid by resting cells. P-16 excreted only trace amounts of 1-hydroxy-2-naphthoic acid and demonstrated linear kinetics in response to 1-hydroxy-2-naphthoic acid concentration. P-15 was found to form thick biofilms on phenanthrene crystals and was characterized by a hydrophobic cell surface, whereas P-16 grew mostly in suspension and was hydrophilic. Neither organism produced significant amounts of biosurfactants when grown on phenanthrene. The implications of these findings for the design of systems to remediate contaminated soil are discussed.
Published Version
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