Abstract
GFP signal intensity revealed similar patterns of GFP absorption in rat and frog proximal tubular cells depending on the amount of the administered protein [1, 3]. At the same time, in frog kidney there were demonstrated some peculiarities of GFP fluorescence distribution associated with a smaller number of nephrons, their intermittent activity and a reduced glomerular filtration under the effect of antidiuretic hormone [2, 3]. Efficiency of the model fluorescent proteins in physiological experiments and a paucity of evidence concerning functional differences between the mammalian metanephros and amphibian mesonephros urged a detailed comparative analysis of protein filtration and absorption in kidneys of rats and frogs. The goal of this investigation was to compare functional parameters of the kidney activity in the above species including the glomerular filtration rate (GFR) and dynamics of GFP and YFP uptake and accumulation by proximal tubular cells during endocytosis. Experiments were carried out on Wistar rat females (Rattus norvegicus) and hibernating brown frog males (Rana temporaria). Highly purified recombinant GFP and YFP were obtained from the Laboratory of protein synthesis mechanisms (Institute of Protein Research RAS, Pushchino, SHORT COMMUNICATIONS
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