Abstract
Publisher Summary This chapter presents the selection of magnetic separators to illustrate differences in approaches to magnetic cell separation. The engineering design of the magnetic cell separators depends on the type of the magnetic particle, available for cell labeling. A highly successful open-gradient type of separators was designed around micrometer-sized Dynabead magnetic particles. Dynabeads were the first magnetic particles used for clinical cell separation applications, and their use has been extended to applications in molecular biology. The chapter describes an example of the Dynabead-Invitrogen separator. The use of colloidal magnetic particles as magnetic labeling reagents offers advantages of their forming stable suspensions and a fast cell binding reaction kinetics. In search for an ideal combination of a colloidal magnetic reagent with an open-gradient magnetic field configuration, a number of designs have been proposed and now are available commercially. One of these designs is based on a concentric, quadrupole magnetic field that fits naturally the cylindrical shape of the laboratory tube, used in combination with magnetic dextran colloid. The other design uses a rectangular magnet assembly that is a source of high field gradients, used in combination of a laboratory tube stand and a customized magnetic colloid.
Published Version
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