Combining urinary microprotein and urinary enzyme for diagnosis renal injury

Abstract

Objective To explore diagnostic basis for early renal lnjuryo Methods Enzyme-linked immunosorbent assay wag used,urine protein icroalbumin,Micoalbuminl,retinol binding protein.Urinary N-acetyle B-D-glucosaminidase was determineby chromatometry.Urinary creatinine was determined with enzymic method.Results The levels of urinary transferrin,miroalbumin and NAG in the controls were Alb/Cr(1.93±0.42(mg/mmol),UPI/Cr 47.61±5.86(mg/mmol),RBP/cr:24.41±3.55(mg/mmol),NAG/Cr.0.96±0.58(u/mmol).The levels of urirary transferrin,microalbumin and NAG in urinary albumin negative groups(diabetes,hypertension,lupus erythematosus,Wethra infection)were higher than those in the controls(P＜0.01),positive rates of determining one or two of the four items were lowerthan that of determining Alb UPl RBP and NAG.together.Determing for items together would raise the positive rate to 92% in diabetes,80%in hypertension,85%in lupus erytematosus,76%in urethra infection respectively.Conclusion It is sensitive and reliable tO detect Alb,UPI,RBP and NAG together for diagnosis of early renal injury. Key words: Kidney; Injuries; Diagnosis; laboratory techniques and procedures