Abstract
Abstract An alternate prepurification procedure was developed for the isolation and purification of azadirachtin, a biologically active limonoid, from neem (Azadirachta indica) seeds by the use of Florisil and droplet counter-current chromatographies. The procedure, combined with the use of preparative high performance liquid chromatography, yielded 364.8 mg of >99% pure azadirachtin from 1.5 kg of Indian neem seeds.
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