Abstract
Xanthomonas oryzae pv. oryzae (Xoo) is a plant pathogen responsible for causing bacterial blight in rice. The immediate alterations in Xoo upon initial contact with rice are essential for pathogenesis. We studied time-resolved genome-wide gene expression in pathogenicity-activated Xoo cells at the transcriptome and proteome levels. The early response genes of Xoo include genes related to cell motility, inorganic ion transport, and effectors. The alteration of gene expression is initiated as early as few minutes after the initial interaction and changes with time. The time-resolved comparison of the transcriptome and proteome shows the differences between transcriptional and translational expression peaks in many genes, although the overall expression pattern of mRNAs and proteins is conserved. The discrepancy suggests an important role of translational regulation in Xoo at the early stages of pathogenesis. The gene expression analysis using time-resolved transcriptome and proteome provides unprecedented valuable information regarding Xoo pathogenesis.
Highlights
Rice (Oryza sativa L.) is the most widely consumed staple food, sustaining two-thirds of the world’s population (Jackson, 2016)
The in vitro assay system recapitulated the initial interaction between Xanthomonas oryzae pv. oryzae (Xoo) cells and damaged rice leaf tissues at the site of infection
Fresh rice leaf extracts (RLX) were prepared by grinding the leaves of a Xoo-susceptible rice cultivar (Milyang 23) in liquid nitrogen and added to a Xoo cell culture in the mid-exponential phase
Summary
Rice (Oryza sativa L.) is the most widely consumed staple food, sustaining two-thirds of the world’s population (Jackson, 2016). Oryzae (Xoo) is a causal agent of bacterial blight of rice, which causes severe yield losses of up to 50% in several rice-growing countries (Oliva et al, 2019). The demand for rice is expected to increase by at least 25% by 2030, owing to the Abbreviations: Xoo, Xanthomonas oryzae pv. The Green Revolution has resulted in a shift in rice cultivation, from varied traditional landraces to limited high-yielding varieties, via artificial selection. This has caused the co-evolution of crop pathogens including Xoo with the selected host races in the modern agricultural ecosystem (Quibod et al, 2020)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
