COLISEPTICEMIA INFECTION IN A 20 DAYS OLD BROILER CHICKEN IN TIMUHUN VILLAGE, KLUNGKUNG

Abstract

Colisepticemia is one of poultry infectious disease caused by Avian pathogenic Escherichia coli. The aim of this study was identifying the causative of an infected broiler chickens aged 20 days. The research was conducted at a close house farm in Timuhun Village, Banjarangkan, Klungkung Regency, Bali with a population of 16,000 broiler chickens. The number of sick chickens was 163 and 55 died. The chicken samples were brought to the Veterinary Pathology Laboratory, Udayana Faculty of Veterinary Medicine for necropsy. Organ samples that underwent changes were taken to the Veterinary Bacteriology and Parasitology Laboratory. In bacteriological examination, organ samples were isolated using nutrient agar and eosyne methylene blue agar (EMBA) media. Furthermore, the results of bacterial isolation were identified using triple sugar iron agar (TSIA) media, Simmon Citrate Agar (SCA), Sulfid Indole Motility (SIM), Methyl Red-Voges Proskauer (MR-VP) and testing sugars (glucose and lactose). In the parasitology laboratory, fecal samples were examined using native, sedimentation and flotation methods. The results of the epidemiological examination of the disease, 1% morbidity, 0.3% mortality and 33.7% case fatality rate (CFR). Post-mortem examination found typical anatomical pathology in the form of polyserotysis and fibrin accumulation in the pericardium, perihepatic, pleural and mesentery. The results of the fecal examination did not find any agents and/or worm eggs. Bacterial isolation on EMBA media grew metallic green round colonies with black dots. Gram stain shows short rods and is colored red. Based on the results of pathology, parasitology and bacteriology examinations, the etiology of this cases caused by Avian Pathogenic Escherichia coli (APEC). Elevating sanitation was needed to reduce the cases.