Abstract
In the development of broiler farming in Indonesia, it is necessary to be aware of diseases that often attack livestock, namely Aspergillosis fungal infection. This case study aims to confirm the diagnosis in the death of 30-day-old broiler chickens of male sex who experienced clinical symptoms of lethargy, nasal discharge, shortness of breath, swollen eyelids, and difficulty eating for 3 days so that they were separated from the rest of the chicken population obtained at one of the farms located in Tunjuk Village, Tabanan District, Tabanan Regency, Bali. Examination of case animals included signaling, anamnesis, epidemiological data, clinical symptoms, anatomical pathology and histopathology examination, bacteriology and mycology examination, and parasitology examination. The results of the anatomical pathology examination showed congestion in the brain, petechie in the ventriculus, hemorrhage in the intestines, hemorrhage in the lungs with white nodules and white nodules in the kidneys. Histopathological examination found septa hyphae and conidiophore. In the mycological examination, the case animals identified Aspergillosis in the lung and kidney organs grown on SDA (Sabouraud Dextose Agar) media and observation under a microscope using 10% KOH dye and methylene blue. In the bacteriological examination, samples of lung and intestinal organs were cultured on general Nutrient Agar (NA) media, followed by selective Eosin Methylene Blue Agar (EMBA) media, primary tests, gram staining, biochemical tests and sugar tests until the results showed that there was a secondary infection of Klebsiella sp in the lung organ samples. The results of the examination related to parasites in the feces of case chickens by qualitative methods, namely natif (direct), sedimentation concentration, and buoyancy concentration were confirmed not to be found worm eggs or coccidia. From this case study it can be concluded that the case chicken was infected with Aspergillosis. Prevention of the spread of Aspergillosis should be done by improving farm management such as housing sanitation and good husbandry methods to prevent the risk of livestock experiencing the disease.
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