Abstract
This chapter discusses the cloning of human T-lymphocytes. The cloning efficiency and regulatory kinetics provide valuable data regarding abnormalities of lymphoid populations in autoimmune disease. The chapter describes a study in which the ability of purified peripheral blood T-cells isolated by E-rosette formation to form colonies in the absence of monocytes were determined. Also, the cloning efficiency of MOLT 4 and RAJI cells was compared. The cloning efficiency of PBL from 20 normal volunteers was compared to 14 patients with systemic lupus erythematosus. Patients taking high dose prednisone or cytotoxic agents were excluded from the study. A characterization of cell types revealed markers consistent with those attributable to cells of T-lymphocyte lineage. When the cloning efficiency of cells from normal volunteers is compared with that of MOLT-4 cells and RAJI cells, the colonies are suggested to consist of T-lymphocytes or their precursors. The cloning efficiency of MOLT-4 cells is significantly reduced below that of normal volunteers, suggesting fundamental functional changes in transformed cells.
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