Clinical application of three methods for the detection of brucellosis

Abstract

Objective To detect serum in high risk population, three methods were used and their advantages and disadvantages was compared. Methods In accordance with the surveillance standard for brucellosis (GB 16885-1997) and diagnostic criteria (WS269-2007) in the prescribed method, rose-bengal plate agglutination test (RBPT), standard-tube agglutination test (SAT) and enzyme linked immunoassay assay (ELISA) were used to detect and analysis of its diagnostic significance in the high risk population of sheep farm of Guoyang county in Anhui province. Results The positive rates of RBPT, SAT and ELISA were 19.1%, 12.1% and 16.3% in 257 blood samples, respectively.Compared to SAT, the sensitivity, specificity, accuracy and the area under the ROC curve of RBPT were 88.5%, 91.8%, 91.4%, 0.81, respectively, which of ELISA were 93.9%, 95.1%, 94.9%, 0.88. Conclusion The sensitivity, specificity, accuracy and the area under the ROC curve of ELISA were higher than those of other methods.Proper method, early surveillance and effective technology can help to control the occurrence and epidemic of in the actual test work promotion. Key words: Brucellosis; Rose-bengal plate agglutination test; Standard-tube agglutination test; ELISA method; Detection