Abstract
Abstract 2-Fluoro-l-erythro-[3,4,5,6-14C4]citrate is actively transported in Bacillus subtilis cells by the citrate-Mg2+ transport system. The kinetic data for uptake of the analogue are very similar to those previously determined for citrate transport. Citrate and 2-fluoro-l-erythro-citrate mutually inhibit their uptake in a competitive manner. Only insignificant metabolism of the analogue was detected after uptake into cells induced for citrate-Mg2+ transport. 2-Fluoro-l-erythro-citrate induces the citrate-Mg2+ transport system with a delay of about one generation time relative to induction caused by citrate. The fluoro analogue probably causes indirect induction of citrate-Mg2+ transport by inhibition of aconitase (EC 4.2.1.3) and subsequent accumulation of endogenous citrate. 2-Fluoro-l-erythro-citrate strongly inhibits aconitase in extracts of B. subtilis cells. B. subtilis mutants resistant toward growth inhibition by 2-fluoro-dl-erythro-citrate in ribose minimal medium were isolated and found to be defective in citrate-Mg2+ transport. These mutants can still grow, albeit very slowly, on plates containing citrate minimal medium. Preliminary results suggest that a second system exists in B. subtilis cells which transports citrate slowly and with lower affinity than the previously described citrate-Mg2+ transport system.
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