CircTHBS1 targeting miR-211/CCND2 pathway to promote cell proliferation and migration potential in primary cystitis glandularis cells.

Yue Ma,Honggang Shao,Zhengfei Shan,Ying Liu,Yupeng Xin,Kai He,Shichun Jiang,Yaodong Wang

doi:10.1042/bsr20201164

Yue Ma, Honggang Shao + Show 6 more

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https://doi.org/10.1042/bsr20201164

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Abstract

The pathogenesis of cystitis glandular (CG) is unclear, but it is generally considered to be a neoplastic lesion of urothelial hyperplasia formed by long-term chronic stimulation. There is growing evidence that circRNAs play important roles in a variety of cellular processes. However, there are few reports on the role and molecular mechanism of circRNA in CG. In the present study, we first isolated primary cells from CG tissues and adjacent normal tissues. Further experiments showed that CircTHBS1 was up-regulated in primary CG cells (pCGs). The results of CCK-8 showed that the overexpression of CircTHBS1 promoted the viability of pCGs, while the deletion of CircTHBS1 reduced the cell viability. Knocking out CircTHBS1 also inhibited the migration of pCGs. In addition, we demonstrated that CircTHBS1 played a role in the adsorption of miR-211 by “sponge” in pCG. In turn, miR-211 can directly target CYCLIN D2 (CCND2) 3′UTR to perform its function. Finally, we confirmed the role and mechanism of CircTHBS1/miR-211/CCND2 regulation axis in pCGs. In summary, our study is the first to reveal the role and underlying mechanism of CircTHBS1 in CG, providing a potential biomarker and therapeutic target for human CG.

Highlights

Cystitis glandular (CG) is a proliferative lesion of the bladder mucosa [1,2,3]
Experimental results show that CircTHBS1 is up-regulated in primary CG cell (pCG) compared with pNCs (Figure 1A)
Cell viability assay results showed that knockout of CircTHBS1 inhibited the viability of pCGs (Figure 1D), while overexpression of CircTHBS1 promoted the viability of pCGs (Figure 1E)

Summary

Introduction

Cystitis glandular (CG) is a proliferative lesion of the bladder mucosa [1,2,3]. In recent years, the incidence of cystitis glandular has been increasing year by year. It is important to study the pathogenesis of glandular cystitis [5]. Studies have shown that circRNA is involved in the transcription and expression of genes through many pathways and play an important role in physiological processes such as cell cycle or aging. More and more studies show that circRNA plays an important role in the occurrence and development of diseases including inflammation and cancer. MicroRNA (miRNA) is a type of single-stranded non-coding small-molecule RNA with a length of 21–23 nucleotides [6]. It regulates gene expression at the post-transcriptional level, thereby realizing the regulation of physiological and pathological processes such as tissue development, individual development, and tumorigenesis. Recent studies have found that microRNA-211 (miR-211) is closely related to the occurrence and development of various diseases, may License 4.0 (CC BY)

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