Abstract
In this study, a chromatography-free purification process through a three-step tandem cavitation fractional precipitation (CFP) was developed to obtain high-purity (+)-dihydromyricetin. Ultrasound and negative pressure were introduced simultaneously into three types of precipitation solutions (acetone-water, methanol-water, and ethanol-water) and precipitation was performed for 30 min. The purity of (+)-dihydromyricetin after the three-step tandem CFP was >98 % and the overall yield was >90 %. In addition, the impurity removal patterns at each precipitation step were observed through reverse phase-high performance liquid chromatography analysis, and the chemical structure of (+)-dihydromyricetin finally purified through the three-step tandem CFP was confirmed through Fourier-transform infrared spectroscopy analysis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
