Abstract
Non-histone proteins were extracted from chromatin of citric acid-isolated, and NPT-isolated (cemulsol-12) salivary gland nuclei and citric acid-isolated midgut nuclei of Drosophila hydei. The migration pattern on sodium dodecyl sulphate-urea polyacrylamide gels of the two types of salivary gland chromatin extracts was significantly different. Also the non-histone proteins of citric acid salivary gland and midgut chromatin gave a consistently different pattern. The DNA prepared from chromatin of both types of nuclei displayed a hyperchromicity of 38–39 %, a melting temperature of 83.5 °C and a buoyant density of 1.700 g/ml corresponding to a molar fraction ( G+C ) of 41 %. In vitro transcription of chromatin with Escherichia coli polymerase at 37 °C attained a plateau after 45 min. Saturation hybridization with in vitro synthesized RNA was obtained with 5–6 μg RNA/μg DNA. RNA transcribed from salivary gland chromatin and midgut chromatin displayed a hybridization percentage of 2.2 and 1.6, respectively.
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