Chitosan-coated magnetic solid lipid nanoparticles for controlled release of letrozole

Abstract

In present study, magnetic solid lipid nanoparticles (MSLNs) with chitosan (CHI) coating were defined, and the rapid delivery of Letrozole (LTZ) to cancer cells by both active and passive targeting were evaluated. LTZ loaded MSLNs were fabricated using the modified solvent evaporation-ultrasonic combination method. The physicochemical parameters were optimized to achieve a suitable drug delivery system. Afterward Fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC) and X-ray diffraction (XRD) used as characteristic analyses. DPPH free radical scavenging test was carried out to evaluate the antioxidant capacities of LEC (Lecithin), MSLNs, LTZ, LTZ + MSLNs, and BHT (Butylated hydroxytoluene). DPPH test indicated similar antioxidant activities for LTZ + MSLNs and BHT. For the determining of anti-breast cancer potentials of LEC, MNPs, LTZ, and LTZ + MSLNs, MTT assay was used on the normal cell line (HUVEC), breast adenocarcinoma (MCF7), breast carcinoma (Hs 578Bst), infiltrating ductal cell carcinoma (Hs 319.T), infiltrating lobular carcinoma of breast (UACC-3133), inflammatory carcinoma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453) cell lines. The desired and favorable particle size and surface charge nanocarrier for targeting cancer cells was obtained. The effect of LFPMF on drug release of the drug delivery system distinctly demonstrated. The findings showed that MSLN loaded with LTZ increased chemotherapy efficiency. LTZ encapsulated MSLN can act as a suitable and promising candidate for targeted and controlled therapy for cancer.