Chemo-enzymatic approaches for the creation of novel chiral building blocks and reagents for pharmaceutical applications

Abstract

This paper describes three recent examples of innovative biocatalyst applications and technology developments from our laboratory giving access to useful chiral building blocks (examples 1 and 2) and chiral reagents (example 3). 1.Preparative to larger scale synthesis of enantiomerically pure chiral arylalkylamines using whole cell biotransformations with microorganisms containing novel enantioselective amidohydrolases.2.Preparative to larger scale chemo-enzymatic synthesis of d- and l-tert-leucines using the very cheap commercial bulk enzyme Alcalase.3.Preparative scale chemo-enzymatic synthesis of rare and expensive inositol phosphates, including 5′-TAMRA-labeled (P1-tethered) d-PIP3, using commercial lipase preparations.