Abstract
The phenomenon of chemiluminescence /CL/ was used for the estimation of granulocytes phagocytic activity. CL was measured in the whole blood of 30 septic neonates and 20 healthy ones after stimulation with 3 different doses of latex (20, 50, 200μl). Peak CL height, time to peak and total light emission were measured within the period of 15 minutes. It was shown that CL pattern varied according to the pathogen. Peak height and time to peak in 20 infants with sepsis caused by Staphylococcus epidermidis were reduced in comparison to 20 healthy newborns (p<0.01). In 2 cases of E.coli septicaemia the quenching of CL response was observed. Moreover, the discriminant analysis was applied to the results of CL in both groups. The most discriminant parameters among the infants with hiperleukocytosis appeared to be: time to peak for 50 μl (F=6.8; total light emission for the stimulation both with 50 μl (F=5.8) and 200 μl of latex (F=5.5). Based on the above results we concluded that CL may be used as a very quick mioromethod in early diagnosis of neonatal septicaemia.
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