Abstract
Background: Stachys (Lamiaceae) is a genus with varied effects and applications in traditional medicine. The essential oil (EO) of different species of genus Stachys has received much attention in different studies. According to previous evidence, the components of EO in different species have diversity although there is no previous data regarding investigating Stachys fruticulosa for EO. Considering the importance of the plants of genus Stachys, vast types of compounds in the EO of the genus, the aim of the present study was to evaluate the components of EO of S. fruticulosa M. Bieb. Methods: The EO of the aerial parts from S. fruticulosa was obtained by hydrodistillation and then the oil was analyzed by gas chromatography/mass spectrometry (GC/MS) and 95% of the oil (17 components) was identified accordingly. The identity of the components was assigned by comparing their mass spectra and retention indices with those of authentic samples. Results: Most oil components were α-terpinyl acetate (24.6%), 3-n-Butyl phthalide (20.5%), p-cymene (18.2%), and β-phellandrene (18.2%). Conclusion: The components of essential oil from S. fruticulosa were identified for the first time, and these substances may be responsible for the biological effects of these essential oils.
Highlights
In the flora of Iran, genus Stachys is represented by 34 species while its number is about 300 species in the world
Gas chromatography (GC) and GC-mass spectrometry (MS) analyses enabled the identification of 87 compounds representing 90.8% of the total oil
The obtained extracts from the aerial parts of S. fruticulosa were examined for its antibacterial activities against G+ and G- strains and the results revealed that S. fruticulosa methanolic extract inhibited the growth of Staphylococcus aureus and Bacillus cereus [13]
Summary
In the flora of Iran, genus Stachys is represented by 34 species while its number is about 300 species in the world. According to another report [4], the water distilled EO of the aerial part of S. pubescence was rich in fatty acids like hexadecanoic and linoleic acids in addition to benzaldehyde and spathulenol whereas the steam distilled oil of the plant contained hexadecanoic acid, spathulenol, and eugenol. Both oils were rich in fatty acids (36.6% and 27.9%, respectively). Methods: The EO of the aerial parts from S. fruticulosa was obtained by hydrodistillation and the oil was analyzed by gas chromatography/mass spectrometry (GC/MS) and 95% of the oil (17 components) was identified .
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