Abstract
1. 1. Two deoxyribonucleoprotein fractions (deoxyribonucleoprotein I and deoxyribonucleoprotein II) were isolated from Yoshida ascites hepatoma cells and from normal rat liver according to the method described by Atchley and Bhagavan. The chemical composition and metabolic activity of the constituent DNA, RNA and histone were studied. 2. 2. Deoxyribonucleoprotein II contained more metabolically active DNA and RNA as compared with deoxyribonucleoprotein I, but its protein portion was less active than that of deoxyribonucleoprotein I, as shown by the incorporation of labeled precursors. 3. 3. Deoxyribonucleoprotein II had a lower N P ratio than that of deoxyribonucleoprotein I. The histone fraction, extracted with dilute acid from deoxyribonucleoprotein II (histone II), showed a lower ratio of total basic amino acids total acidic amino acids than histone I (extracted from deoxyribonucleoprotein I). Histone II was composed mainly of protein corresponding to the run-off peak fraction obtained from chromatography on an Amberlite IRC-50 column and typical histone sub-fractions represented rather minor components. In contrast, histone I contained all of the typical histone sub-fractions and a smaller amount of the run-off peak fraction. 4. 4. The histones and their sub-fractions were further analyzed by means of disc electrophoresis and differences were noted between the sub-fractions of histone I and histone II. Histone II seemed to lack certain bands corresponding to fraction I and fraction III + IV obtained in work by Luck et al.
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