Abstract
In the present study silver nano-particles were synthesised by green synthesis method using fenugreek leaves extract, provides necessary bioactive for reduction of silver ions to silver nanoparticles. The sharp bands of silver nanoparticles were observed around 432 nm. Synthesis process was further optimized for pH, extraction temperature, Ag ion concentration, extract volume and extraction time on the basis of absorbance and average particles size respectively. FTIR analysis showed presence of corresponding peaks of O–H stretching and N–H amide groups. FESEM images revealed the structure and morphology of spherical AgNPs of average size 30 nm. The antibacterial activity of thus synthesized AgNPs was then tested against S. aureus, E. coli, P. aeruginosa and V. cholera and found synthesized AgNPs have higher inhibitory action against P. aeruginosa compared to other bacteria. The larger size of nano-particles is less active than smaller size of nano-particles due to small surface area.
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