Abstract
The Green Synthesis method is widely developed due to its environmentally friendly, cost-effective, and easy application for nanoparticle-scale synthesis. Among all metal nanoparticles, silver nanoparticles are the most utilized products in the field of nanotechnology. Biomolecules contained in plant extracts can reduce the size of silver particles to nano size. This study aims to determine the antibacterial properties and activity of silver nanoparticles synthesized with the ethanol extract of mahkota dewa leaves. The formation of silver nanoparticles is monitored with UV-Vis absorption and its change in color. Parameters evaluated are shape, size, particle size distribution, composition, metal residue, and a functional group of nanoparticles, using Scanning Electron Microscopy (SEM), Particle Size Analyzer (PSA), X-Ray Diffraction (XRD), and Fourier Transform InfraRed (FTIR) instrument. The research on UV-Vis color and absorption show black silver color with the wavelength of 450-465nm. The characterization result shows spherical-shaped silver nanoparticle. Furthermore, PDI best value on concentration of 0.125% is 0,221±0,0482 with average particle size of 130,300±12,6858 nm. The diffraction pattern of silver nanoparticle with XRD test indicates that the nanoparticles contain the silver component. Antibacterial activity test shows that silver nanoparticles have a greater inhibition zone than AgNO3, and 0.125% ethanol extract of mahkota dewa leaves against Escherichia coli and Staphylococcus aureus. From the results of the study, it can be concluded that the ethanol extract from mahkota dewa leaves can be used as a bioreductor agent to produce silver nanoparticles which have greater antimicrobial activity compared to Ag and ethanol extract from mahkota dewa leaves.
Highlights
Diabetes mellitus is a chronic disease in which complication could affect the quality of the patient's life
This is because agglomeration occurs at high temperatures and particles formed in large size
The same study was conducted on the ethanol extract of mahkota dewa leaves in which the results show that the extract had weak activity against Staphylococcus aureus and Escherisia coli bacteria at a concentration of 10 mg/ml (Matsjeh et al, 2015)
Summary
Diabetes mellitus is a chronic disease in which complication could affect the quality of the patient's life. WHO has predicted an increase in diabetics from 6.4% (285 million) in 2010 to 7.7% (439 million) in 2030 (Mihardja et al, 2014). Diabetic foot ulcer (DFU) is one of the chronic complications in diabetes mellitus caused by the presence of neuropathy and vascular disorders in the legs, and these complications could have a large long-term impact on the patient's morbidity, mortality, and quality of life (Sing et al, 2013). The most common pathogen that can be seen is gram-positive aerobic cocci, Staphylococcus aureus, and βhemolytic streptococci. Resistance or multiresistance has become a major problem in the health sector, for instance, the resistance of Staphylococcus aureus to methicillin and Candida albicans to fluconazole.
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