Abstract
Proton T2 relaxation is a useful parameter for investigating the motion of and interaction between dried proteins and water molecules. This study aimed to investigate the relationship of proton T2 relaxation with aggregation and water hydration or dehydration in five bovine lactoferrin powders (LFs) before and after storage at 40 °C or 50 °C. Using time-domain nuclear magnetic resonance (TD-NMR), we detected T2short and T2long with different relaxation times. The two fractions and their average T2 relaxation time (T2) were used for analysis. Average T2 was positively correlated with the pre-storage water content. For the 40°C-storage test, T2 increased in all LFs; this increase can be explained by the monomer loss rate and change in water content. Conversely, for the 50°C-storage test, the change in T2 was correlated with the change in water content. Furthermore, the tendency for change in T2 showed variation with the monomer ratio and water content before storage. Thus, this study provides a novel insight that the factors contributing to the change in T2 during storage vary with storage temperature. In addition, we suggested that the T2 relaxation time was not sufficient as a predictor of susceptibility to aggregation.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call