Abstract
Abstract Callus cultures of Podophyllum hexandrum were initiated and established from juvenile explants isolated from axenic seedlings and mature explants isolated from 1 year old plants on B5 basal medium (at half strength) and modified MS medium supplemented with growth regulators. For initiating cell lines, cell suspension cultures were established with selected callus lines in B5 basal medium supplemented with growth regulators and cell lines were established by cell plating technique. Eight cell lines (RC1, RC2, RC3, RC4, RC5, RC6, RC7 and ZE) maintained as callus stocks in the laboratory have been characterized and compared on the basis of growth rate, morphology, podophyllotoxin content, ploidy, karyotype analysis and RAPD analysis. Morphologically the cell lines were of six different types like globular and dark brown, friable with rough surface and light brown, compact and yellow, compact and creamish, compact and dark brown in colour and creamish and highly friable in nature. The cell lines had ...
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