Characterization of Newly Developed Wheat/Barley Introgression Lines in Respect of Aluminium Tolerance

Eva Darko,Márta Molnár-Láng,Beáta Barnabás

doi:10.4236/ajps.2012.310176

Abstract

The Al tolerance of newly developed wheat/barley disomic addition, substitution and translocation lines carry chromosomes of three different barley cultivars was evaluated by comparing the root growth in solution containing 75 μM AlCl3 at pH 4.0 to that of known Al-tolerant and sensitive wheat genotypes. The wheat Asakaze komugi, barley Manas cultivars and their hybrid derivatives were found to have high levels of Al tolerance. The wheat line Mv9kr1, barley cultivar Igri and progenies of the hybrids were sensitive to Al. In most cases, the Al tolerance of the wheat/barley introgression lines derived from Al-sensitive wheat Mv9kr1 and barley Betzes with moderate Al tolerance was similar to that of the wheat parents, but the 2DS.2DL-1HS translocation line of Mv9kr1/Betzes exhibited more intensive root growth, while accumulating less Al than the parental lines. This indicates that either the lack of the distal part of chromosome 2DL or the presence of the distal part of 1HS improved the Al tolerance level.

Highlights

Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) are important cereals worldwide
The Al tolerance of several wheat/ barley disomic addition, substitution and translocation lines and that of the parental genotypes was studied under hydroponic conditions and compared to that of wheat genotypes (Atlas 66, Chinese Spring and Scout 66) with known levels of tolerance
This confirms that Al toxicity is and barley Betzes (d) parents grown in nutrient solution containing 75 μM AlCl3 at pH 4.0 determined by morin staining

Summary

Introduction

Wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) are important cereals worldwide. The first successful hybridization between wheat and barley was reported by Kruse [1] and not much later a set of wheat/barley addition lines was produced [2]. The addition lines were produced from Chinese Spring wheat and Betzes barley cultivars which have high crossability in intergeneric crosses but are unsatisfactiory from the agronomic point of view. Very few new hybrid combinations have been reported from wheat × barley crosses since and in most cases no backcross progenies were developed [3,4]. The agronomic characters of the new wheat/barley hybrid derivatives are not known experiments were carried out to analyse the tolerance of these lines to various environmental stress factors [9,10]. The tolerance level of the wheat/barley addition and translocation lines and their parental genotypes to Al toxicity has not been yet investigated, with the exception of the barley Igri which has low to moderate Al tolerance [11]

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Conclusion