Registration of DGE‐2, a Durum Wheat Disomic Alien Substitution Line 1E(1A) Involving a Diploid Wheatgrass Chromosome

Abstract

The durum wheat (Triticum turgidum L., 2n = 2x = 28; AABB) alien disomic substitution 1E(1A) line DGE‐2 (Reg. No. GS‐171,PI 663216) was developed by the USDA‐ARS, Cereal Crops Research Unit, Northern Crop Science Lab, Fargo, ND and released in 2011. Earlier, we produced and released a durum alien disomic addition line DGE‐1 (2n = 30; PI 645483) that incorporated chromosome 1E of a diploid wheatgrass, Lophopyrum elongatum (Host) Á. Löve (2n = 2x = 14; EE). We crossed DGE‐1 with ‘Langdon’ (CItr 13165) disomic substitution line 1D(1A) and produced F1 hybrid derivatives with chromosomes 1A, 1D, and 1E in a single dose. We selfed these hybrid derivatives five times and screened each generation for homoeologous group‐1 chromosomes 1A and 1B. At the same time, these hybrid derivatives were tested for the presence of 1E and absence of 1D. Using the chromosome‐specific molecular markers Xwmc333 for identifying chromosome 1A, Xwgm18 for 1B, Xwmc147 for 1D, and Xedm17 for 1E, we isolated a stable disomic alien substitution line 1E(1A), which has been released as a new genetic stock, DGE‐2. The pedigree of DGE‐2 is DGE‐1 (2n = 28 + 2; PI 645483)/Langdon 1D(1A)*5. This unique disomic alien substitution line DGE‐2 has 2n = 28 chromosomes, in which the wheatgrass chromosome pair, 1E, compensates for the 1A pair of the durum cultivar Langdon. The disomic substitution has regular meiosis and is fertile. With a unique chromosomal constitution and a unique allele Glu‐E1b, DGE‐2 may be useful in basic research.