Registration of DGE‐3, a Durum Wheat Disomic Substitution Line 1E(1B) Involving a Wheatgrass Chromosome

Abstract

Durum wheat (Triticum turgidum L., 2n = 4x = 28; AABB genomes) alien disomic substitution 1E(1B) line DGE‐3 (Reg. No. GS‐172, PI 665473) was developed by the USDA‐ARS, Northern Crop Science Lab, Cereal Crops Research Unit, Fargo, ND and released in 2012. It was derived from the durum alien disomic addition line DGE‐1 (2n = 28 + 2) having 28 durum chromosomes and the chromosome pair 1E of diploid wheatgrass, Lophopyrum elongatum (Host) Á. Löve. The pedigree of DGE‐3 is PI 645483/‘Langdon’ 1D(1B)*5. By crossing DGE‐1 (PI 645483) with durum disomic substitution line 1D(1B), we produced hybrid derivatives with univalents of chromosomes 1B, 1D, and 1E. We selfed the hybrid derivatives five times and screened each generation for durum homoeologues 1A and 1B. We tested these hybrid derivatives for the presence of the alien chromosome 1E and for the absence of 1D. Using the chromosome‐specific molecular marker Xwmc333 for identifying chromosome 1A, Xwgm18 for 1B, Xwmc147 for 1D, and Xedm17 for 1E, we isolated a durum disomic alien substitution line 1E(1B), which is fertile and stable and has been released as a new genetic stock, DGE‐3. This substitution line has 2n = 28 chromosomes that consist of 26 durum chromosomes plus the wheatgrass chromosome pair, 1E, which compensates for the missing 1B pair of Langdon. The disomic substitution forms 14 bivalents and is meiotically regular and fertile.