Abstract
Chemiluminescence reaction efficiently produces light, and that is where its scientific importance lies. For investigating the reaction in live fireflies, we introduce a few protocols that exert light emission at different temperatures and in different color sectors. From the changes in the peak position and the duration of flashes, the light can be characterized. As the firefly emits in green, yellow, and red color sectors, three color-filters are used for getting emissions in these three regions. Emission spectra are recorded in a high-resolution spectrometer, and flashes are obtained in an oscilloscope, after amplification in a photo multiplier tube, in the range of temperature 20-40°C.
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