Characterization of Diaporthe species on Camelliaoleifera in Hunan Province, with descriptions of two new species.

Qin Yang,Jie Tang,Guo Y Zhou

doi:10.3897/mycokeys.84.71701

Abstract

Tea-oil tree (Camelliaoleifera Abel.) is an important edible oil woody plant with a planting area over 3,800,000 hectares in southern China. Species of Diaporthe inhabit a wide range of plant hosts as plant pathogens, endophytes and saprobes. At present, relatively little is known about the taxonomy and genetic diversity of Diaporthe on C.oleifera. Here, we conducted an extensive field survey in Hunan Province in China to identify and characterise Diaporthe species associated with tea-oil leaf spots. As a result, eleven isolates of Diaporthe were obtained from symptomatic C.oleifera leaves. These isolates were studied by applying a polyphasic approach including morphological and phylogenetic analyses of partial ITS, cal, his3, tef1 and tub2 gene regions. Two new Diaporthe species (D.camelliae-oleiferae and D.hunanensis) were proposed and described herein, and C.oleifera was revealed to be new host records of D.hubeiensis and D.sojae. This study indicated there is a potential of more undiscovered Diaporthe species from C.oleifera in China.

Highlights

Tea-oil tree, Camellia oleifera Abel., is a unique woody edible oil species in China, mainly distributed in the Qinling-Huaihe River area
The topologies resulting from Maximum Likelihood (ML) and Bayesian Inference (BI) analyses of the concatenated dataset were congruent (Fig. 1)
Diaporthe camelliae-oleiferae and D. hunanensis are new to science based on the distinct and well-supported molecular phylogenetic placement with their closest described relatives

Summary

Introduction

Tea-oil tree, Camellia oleifera Abel., is a unique woody edible oil species in China, mainly distributed in the Qinling-Huaihe River area. It has a long history of cultivation and utilization for more than 2300 years since ancient China (Zhuang 2008). Hunan Province leads the country in C. oleifera production with the average of 3.3~40,000 hm to expand the cultivation area every year (Tan et al 2018). By the end of 2017, the cultivation area of C. oleifera reached 1.4 million hm, tea oil 290100 tons, and output value of 35 billion yuan (Tan et al 2018). The development of C. oleifera industry is of great significance for the economic development of Hunan Province and the poverty alleviation of local farmers

Objectives

Methods

Results

Conclusion