Abstract
Diaporthe (Diaporthaceae, Diaporthales) is a common fungal genus inhabiting plant tissues as endophytes, pathogens and saprobes. Some species are reported from tree branches associated with canker diseases. In the present study, Diaporthe samples were collected from Alnusglutinosa, Fraxinusexcelsior and Quercusrobur in Utrecht, the Netherlands. They were identified to species based on a polyphasic approach including morphology, pure culture characters, and phylogenetic analyses of a combined matrix of partial ITS, cal, his3, tef1 and tub2 gene regions. As a result, four species (viz. Diaporthepseudoalnea sp. nov. from Alnusglutinosa, Diaporthesilvicola sp. nov. from Fraxinusexcelsior, D.foeniculacea and D.rudis from Quercusrobur) were revealed from tree branches in the Netherlands. Diaporthepseudoalnea differs from D.eres (syn. D.alnea) by its longer conidiophores. Diaporthesilvicola is distinguished from D.fraxinicola and D.fraxini-angustifoliae by larger alpha conidia.
Highlights
IntroductionPhomopsis) is the type genus of Diaporthaceae in Diaporthales, commonly occurring as plant endophytes, pathogens and saprobes (Udayanga et al 2014, 2015; Guarnaccia et al 2017, 2018a, 2018b; Tibpromma et al 2018; Yang et al 2020; Dissanayake et al 2020; Jiang et al 2021)
The topologies resulting from Maximum Likelihood (ML) and Bayesian Inference (BI) analyses of the concatenated dataset were congruent (Fig. 1)
CFCC 54191 and M79 from Fraxinus excelsior and CFCC 54190 and M2A from Alnus glutinosa represent two new species which are here described as D. silvicola and D. pseudoalnea, respectively
Summary
Phomopsis) is the type genus of Diaporthaceae in Diaporthales, commonly occurring as plant endophytes, pathogens and saprobes (Udayanga et al 2014, 2015; Guarnaccia et al 2017, 2018a, 2018b; Tibpromma et al 2018; Yang et al 2020; Dissanayake et al 2020; Jiang et al 2021). The asexual morph is characterized by ostiolate conidiomata, with cylindrical phialides producing up to three types of hyaline, aseptate conidia (Udayanga 2011; Gomes et al 2013; Yang et al 2018), and was previously classified as Phomopsis. Species of Diaporthe are known to cause plant diseases including dieback, canker, leaf spot, fruit rot, pod blights and seed decay. (Udayanga et al 2014), while Daporthe lithocarpi caused leaf spot disease of Castanea henryi in China (Jiang et al 2021). Diaporthe constrictospora and an additional 11 species were isolated as saprobes from dead wood in karst formations in China (Dissanayake et al 2020)
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